Development of loop-mediated isothermal amplification assay for detection of human coronavirus-NL63
نویسندگان
چکیده
منابع مشابه
development of a loop-mediated isothermal amplification assay for rapid detection of yersinia enterocolitica via targeting a conserved locus
background and objectives: loop-mediated isothermal amplification is a novel nucleic acid amplification assay providing as a simple diagnostic tool for rapid identification of microbial diseases in developing countries. in this study, a lamp assay was established for yersinia enterocolitica , a leading cause of acute enterocolitis in young children. materials and methods: lamp assay was establi...
متن کاملRapid detection of infectious bursal disease by loop-mediated isothermal amplification for field analysis
Infectious bursal disease (IBD) is an immunosuppressive, acute and highly contagious illness of growing-poultry stock infected with infectious bursal disease virus (IBDV). It is common in Pakistan, causing potential economic losses throughout the year. The objective of the study is to propose a rapid, sensitive and specific diagnostic tool, and compare it with existing commonly used reverse tra...
متن کاملdevelopment and assessment of loop-mediated isothermal amplification (lamp) assay for the diagnosis of human visceral leishmaniasis in iran.
background: parasitological methods for the diagnosis of visceral leishmania-sis (vl) require invasive procedures, so serological and molecular approaches have been developed but are not generally applicable in the field. we evaluated a loop mediated isothermal amplification (lamp) assay using blood from vl pa-tients and compared it to nested pcr. methods: forty-seven subjects with clinical fea...
متن کاملDevelopment of a loop-mediated isothermal amplification assay for rapid detection of capripoxviruses.
Sheep pox (SP), goat pox (GP), and lumpy skin disease (LSD), caused by capripoxviruses (CaPVs), are economically important diseases of sheep, goats, and cattle, respectively. Here, we report the development of a loop-mediated isothermal amplification (LAMP) assay for rapid detection of CaPVs. LAMP primers were designed to target a conserved gene encoding the poly(A) polymerase small subunit (VP...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2011
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2011.04.024